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Synthetic polymer sulphonated polyisoprene as a universal anticoagulant for laboratory testing
Y Kinoshita1, K Ohta, T Yamane
1Department of Clinical and Laboratory Medicine, Osaka City University Medical School, Asahimachi, Osaka, Japan.
Journal of Clinical Laboratory Analysis
|July 25, 2000
Summary
Sulphonated polyisoprene (SPIP) shows promise as a laboratory anticoagulant for hematological, biochemical, and electrolyte analysis. While it causes platelet aggregation, kanamycin addition partially restores platelet counts and maintains reliable measurements.
Area of Science:
- Biochemistry
- Hematology
- Polymer Science
Background:
- Laboratory anticoagulants are crucial for accurate blood sample analysis.
- Existing anticoagulants may have limitations in preserving certain blood components or analytes.
- Synthetic polymers offer potential for novel anticoagulant development.
Purpose of the Study:
- To assess the feasibility of sulphonated polyisoprene (SPIP) as a novel laboratory anticoagulant.
- To compare hematological, biochemical, and electrolyte measurements in SPIP-anticoagulated samples versus standard methods (EDTA and serum).
Main Methods:
- Blood samples were anticoagulated with SPIP and compared to EDTA for hematological parameters.
- Serum samples were compared to SPIP-anticoagulated blood for biochemical and electrolyte analysis.
- The effect of additives like kanamycin, aspirin, and prostaglandin E1 on SPIP anticoagulation was investigated.
Main Results:
- SPIP caused significantly lower platelet counts due to aggregation compared to EDTA.
- Kanamycin addition restored platelet counts to approximately 80% of EDTA levels and improved correlation for most hematological items.
- High correlation coefficients (>0.95) were observed for 15 of 18 biochemical and electrolyte items between serum and SPIP samples.
- Kanamycin addition sometimes decreased correlation coefficients for biochemical and electrolyte measurements.
Conclusions:
- SPIP is a viable candidate for a single-tube anticoagulant for routine laboratory analysis of hematological, biochemical, and electrolyte parameters.
- Further research is needed to optimize kanamycin as an additive to prevent SPIP-induced platelet aggregation.
- Ensuring the reliability of biochemical and electrolyte measurements with SPIP requires further investigation and potential improvements.