Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temperature-sensitive cell mutations that inhibit adenovirus 2 replication.

T Nishimoto, H J Raskas, C Basilico

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Different Epidemiology of Invasive Pulmonary Aspergillosis in Acute Lymphoblastic Leukemia in Comparison With Acute Myeloid Leukemia: Results of a Prospective Multicentric Observational Study of the Rete Ematologica Lombarda.

    Hematological oncology·2025
    Same author

    Author Correction: Direct observations of pure electron outflow in magnetic reconnection.

    Scientific reports·2022
    Same author

    Direct observations of pure electron outflow in magnetic reconnection.

    Scientific reports·2022
    Same author

    High-power laser experiment forming a supercritical collisionless shock in a magnetized uniform plasma at rest.

    Physical review. E·2022
    Same author

    Robustness of large-area suspended graphene under interaction with intense laser.

    Scientific reports·2022
    Same author

    Cyclic nigerosyl-1,6-nigerose-based nanosponges: An innovative pH and time-controlled nanocarrier for improving cancer treatment.

    Carbohydrate polymers·2018
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Temperature-sensitive mutants of hamster cells (BHK-21) reveal critical cell cycle roles in adenovirus replication. Specific mutations impairing G1 phase arrest significantly reduce virus production, highlighting early replication dependencies.

    Area of Science:

    • Virology
    • Cell Biology
    • Molecular Biology

    Background:

    • Adenovirus replication is a complex process dependent on host cell machinery.
    • Temperature-sensitive mutants offer valuable tools to dissect essential host-virus interactions.

    Purpose of the Study:

    • To investigate the role of specific host cell functions, identified through temperature-sensitive mutants, in supporting adenovirus 2 replication.
    • To determine the precise stage of the cell cycle and viral life cycle affected by these mutations.

    Main Methods:

    • Utilized five temperature-sensitive growth mutants of the BHK-21 hamster cell line.
    • Compared adenovirus 2 multiplication yields at permissive (33°C) and restrictive (39°C) temperatures.
    • Conducted shift-up experiments and assessed viral DNA synthesis.

    Related Experiment Videos

    Main Results:

    • Three mutants (ts T22, ts T23, ts AF8) showed a significant reduction (99%) in adenovirus 2 production at 39°C compared to 33°C.
    • Viral DNA synthesis was reduced by at least 95% in these mutants at the restrictive temperature.
    • ts AF8, a G1-arrest mutant, demonstrated thermosensitivity for the first 40 hours of infection, impacting early replication events.
    • ts T22 and ts T23 showed thermosensitivity for the first 3-4 hours, with virus yield being multiplicity-dependent.

    Conclusions:

    • The cell cycle function affected in ts AF8 is crucial for early adenovirus 2 replication, post-entry and pre-DNA synthesis.
    • Specific host cell functions, particularly those related to the G1 phase, are essential for efficient adenovirus replication.