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PCR based diagnosis of 21-hydroxylase gene defects in Slovak patients with congenital adrenal hyperplasia
L Pinterova1, M Garami, Z Pribilincova
1Institute of Experimental Endocrinology, Bratislava, Slovakia, ueenpint@savba.savba.sk
Objective:
To analyse 21-hydroxylase gene for 8 most common mutations in patients with salt-wasting type of congenital adrenal hyperplasia.
Methods:
Allele specific PCR performed on 8 salt-wasting CAH patients and their 23 healthy relatives.
Results:
Two patients were homozygous for 8 bp deletion in exon 3, while 6 patients were homozygous for intron 2 splice mutation. Mutant allele for splice mutation was found also in both parents of patients with this type of mutation.
Conclusions:
These preliminary results show that only two mutations, 8 bp deletion in exon 3 and splice mutation in intron 2, were present in this group of Slovak patients with salt-wasting type of congenital adrenal hyperplasia.
