Related Experiment Videos
Identification of type 4 fimbriae in Actinobacillus pleuropneumoniae
Y Zhang1, J M Tennent, A Ingham
1CSIRO Animal Health, Australian Animal Health Laboratory, Private Bag 24, 3220, Geelong, Vic., Australia. yamei.zhang@dah.csiro.au
FEMS Microbiology Letters
|July 29, 2000
Abstract:
Type 4 fimbriae have been identified on the cell surface of Actinobacillus pleuropneumoniae by electron microscopy and N-terminal sequencing analysis. A. pleuropneumoniae type 4 fimbrial subunit protein, purified from cell cultures and from outer membrane preparations, reacted with polyclonal antibody raised against type 4 fimbriae of Moraxella bovis on Western blots. N-terminal sequence analysis of the purified 17 kDa type 4 fimbrial subunit protein, named ApfA, revealed the first 12 amino acids to be identical to those of other type 4 fimbrial subunit proteins.