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[Method for preparing monospecific antiserum to fowl plague virus hemagglutinins]
Voprosy Virusologii
|January 1, 1975
Abstract:
A simple method for preparation of monospecific antiserum for the hemagglutinin of fowl plague virus has been developed. It is based on selective inactivation of the enzymatic and antigenic properties of neuraminidase by heating of the virus at 56degrees for 3 hours followed by disruption of the preparation with detergent and removal of the inner proteins by ultracentrifugation. Immunization of animals with such preparations produced antiserum containing considerable amounts of antibody for the hemagglutinin in the absence of antibody for other proteins of fowl plague virus.