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[8-chloro-adenosine induced apoptosis in various human tumor cell lines]
1Institutes of Basic Medical Sciences and Hematology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing.
Objective:
To study the effect of 8-Chloro-adenosine (8CA) on tumor cell growth and apoptosis.
Methods:
Tumor and normal cell lines were treated with 8CA at different concentrations and cell growth was analysed by MTT assay. Cell apoptosis was evaluated by light microscopy, flow cytometry and chromosome DNA fragmentation.
Results:
Treatment with 8CA at 10 mumol/L remarkably inhibited tumor cell growth. HL-60 promyelocytic leukemia cell line was more sensitive to 8CA than MGc-803 gastric mucoid adenocarcinoma cell line. NIH3T3 cells were relatively resistant to 8CA. The IC50 of HL-60 and MGc-803 cells was 1.2 mumol/L and 1.8 mumol/L, respectively, while that of NIH3T3 cells was 12 mumol/L. Further study showed that 8CA induced HL-60 and MGc-803 cell death characterized by apoptotic phenotypes, DNA content changes and chromosome DNA fragmentation.
Conclusion:
8CA is a potential agent for tumor chemotherapy.