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[Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell]
Objective:
To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell.
Methods:
An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine.
Results:
Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line.
Conclusion:
PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.
Insights
Proliferating cell nuclear antigen (PCNA) antisense RNA effectively suppressed human gastric cancer cell growth. This targeted approach inhibited cell proliferation and biosynthesis, offering a potential therapeutic strategy for gastric cancer.
Area of Science:
- Molecular Biology
- Oncology