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Subunits of neurosteroid sulfatase from bovine brain
1Brain Disease Research Center, School of Medicine, Ajou University, Suwon, South Korea.
The Journal of Steroid Biochemistry and Molecular Biology
|August 5, 2000
Abstract:
We have purified the neurosteroid sulfatase (NSS) from Triton X-100 solubilized microsomes of bovine brain about 100-fold. The purified enzyme is composed of two catalytic units (MW: 57 kDa) and two regulatory units (MW: 38 kDa), making it an alpha(2)beta(2) heterotetramer, whose apparent molecular weight was 180 kDa by gel filtration in the presence of Triton X-100.