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Related Experiment Videos

Circulating GH isoforms and GH bioactivity in preterm neonates.

G Radetti1, M Bozzola, S Pagani

  • 1Department of Paediatrics, Regional Hospital of Bolzano, Italy.

Pediatric Research
|August 6, 2000
PubMed
Summary

Human growth hormone (GH) isoforms, including 20-kD GH and 22-kD GH, are elevated in preterm neonates compared to full-term infants and adults. Serum levels of these GH variants decrease in preterm infants by the 15th day of life.

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Area of Science:

  • Endocrinology
  • Neonatal Physiology
  • Molecular Biology

Background:

  • Human growth hormone (GH) exists in various molecular isoforms, with 22-kD GH being predominant and 20-kD GH the second most abundant.
  • The expression and pattern of GH isoforms during the early neonatal period are not well understood.

Purpose of the Study:

  • To investigate the serum levels and bioactivity of 20-kD GH and 22-kD GH in preterm neonates during the early postnatal period.
  • To compare GH isoform levels and bioactivity in preterm neonates with full-term neonates and healthy adults.

Main Methods:

  • Serum samples were collected from 19 preterm neonates on days 4 and 15, 19 full-term neonates on day 4, and 20 healthy adults.
  • Quantification of 22-kD GH using an immunofluorometric assay and 20-kD GH using ELISA.

Related Experiment Videos

  • Measurement of GH bioactivity via the Nb2 cell bioassay.

    • Main Results:

    • Four-day-old preterm neonates exhibited significantly higher serum levels of 20-kD GH, 22-kD GH, and GH bioactivity compared to full-term neonates and adults.
    • A significant decrease in all measured GH parameters was observed in preterm neonates by the 15th day of life.
    • The percentage of the 20-kD GH isoform remained consistent across all groups, indicating changes in total amount rather than relative proportion.

    Conclusions:

    • Preterm neonates demonstrate distinct patterns of GH isoform expression and elevated bioactivity in the early postnatal period.
    • These findings highlight dynamic changes in GH physiology during early development and suggest potential implications for neonatal growth and development.