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Rapid single-tube genotyping of the factor V Leiden and prothrombin mutations by real-time PCR using dual-color

F A van den Bergh1, A M van Oeveren-Dybicz, M A Bon

  • 1Department of Clinical Chemistry, Medisch Spectrum Twente, Hospital Group, PO Box 50000, 7500 KA Enschede, The Netherlands. labmst@euronet.nl.

Clinical Chemistry
|August 6, 2000
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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