Related Experiment Video
Updated: Aug 12, 2026
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
Published on: October 11, 2015
Role of mitotic control in spermatogenesis
M R Gazvani1, E D Wilson, D H Richmond
1Department of Obstetrics and Gynaecology, Liverpool Women's Hospital, Liverpool, UK. m.r.gazvani@abdn.ac.uk
Objective:
To study the correlation between the incidence of sex chromosome aneuploidies in the somatic cells and spermatozoa in karyotypically normal infertile men and fertile donors.
Design:
A prospective, phase two, controlled study.
Setting:
A teaching Hospital Reproductive Medicine and Medical Genetics Units.
Patient(S):
Ten patients with idiopathic oligozoospermia and 10 sperm donors with proven fertility, all with a normal karyotype 46, XY.
Intervention(S):
Multicolor fluorescence in situ hybridization (FISH) of peripheral blood lymphocytes and spermatozoa using a probe cocktail containing the alpha satellite DXZ1 for the X centromere, DYZ1 for the heterochromatic region of the long arm of the Y, and cosmids D21S259, D21S341, and D21S342 for Down syndrome critical region of chromosome 21.
Main Outcome Measure(S):
The incidence of chromosome X, Y, and 21 aneuploidies in peripheral lymphocytes and spermatozoa in both groups.
Result(S):
The incidence of aneuploidies related to chromosomes X, Y, and 21 were significantly higher in peripheral lymphocytes and spermatozoa of infertile men compared with donors. There was a positive correlation between the incidence of chromosome aneuploidies in the somatic cells and sperm in all men.
Conclusion(S):
These findings provide suggestive evidence for the importance of mitosis in spermatogenesis and the role of mitotic instability in unexplained oligozoospermia.
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