Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelial-like cells derived from human CD14 positive monocytes.

B Fernandez Pujol1, F C Lucibello, U M Gehling

  • 1Institute for Molecular Biology and Tumor Research (IMT), Philipps-University, Marburg, Germany.

Differentiation; Research in Biological Diversity
|August 10, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgical stage in the era of molecular profiling of endometrial cancer.

European journal of cancer (Oxford, England : 1990)·2025
Same author

Digital goal management training for cognitive impairment in cervical cancer survivors-a randomized comparison study.

Journal of cancer survivorship : research and practice·2024
Same author

ENGOT-en11/GOG-3053/KEYNOTE-B21: a randomised, double-blind, phase III study of pembrolizumab or placebo plus adjuvant chemotherapy with or without radiotherapy in patients with newly diagnosed, high-risk endometrial cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2024
Same author

NSGO-OV-UMB1/ENGOT-OV30: A phase II study of durvalumab in combination with the anti-CD73 monoclonal antibody Oleclumab in patients with relapsed ovarian cancer.

Gynecologic oncology·2024
Same author

Maintenance olaparib rechallenge in patients with platinum-sensitive relapsed ovarian cancer previously treated with a PARP inhibitor (OReO/ENGOT-ov38): a phase IIIb trial.

Annals of oncology : official journal of the European Society for Medical Oncology·2023
Same author

Pelvic exenteration for vulvar cancer: Postoperative morbidity and oncologic outcome - A single center retrospective analysis.

European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology·2023
Same journal

Direct Reciprocal Repression between Goosecoid and Ventx1.1 Modulates Dorsoventral Patterning in Xenopus.

Differentiation; research in biological diversity·2026
Same journal

Validated CRISPR/Cas9 guide RNAs targeting neurodevelopmental genes in the tunicate Ciona robusta.

Differentiation; research in biological diversity·2026
Same journal

Corrigendum to "Histone H2A deubiquitinase BAP1 is required for human neuronal progenitor cell formation" [Differentiation 149 (2026) 100957].

Differentiation; research in biological diversity·2026
Same journal

Human heel fat pad development before birth.

Differentiation; research in biological diversity·2026
Same journal

Bridging cells: Potential crosstalk between the peripheral and central olfactory sensory system.

Differentiation; research in biological diversity·2026
Same journal

Osteoclast-derived DEL1 promotes pathological bone formation in ankylosing spondylitis by regulating RUNX2 expression in osteoblasts.

Differentiation; research in biological diversity·2026
See all related articles

Human CD14-positive mononuclear cells can transform into endothelial-like cells (ELCs) with angiogenic growth factors. These ELCs express key endothelial markers and form vascular networks, suggesting a link between monocyte and endothelial cell systems.

Area of Science:

  • Cell Biology
  • Hematology
  • Vascular Biology

Background:

  • Mononuclear cells, specifically CD14-positive cells, are known precursors for various cell types.
  • Endothelial cells form the inner lining of blood vessels and play crucial roles in vascular homeostasis.
  • The potential plasticity of mononuclear cells towards endothelial lineages is an area of ongoing research.

Purpose of the Study:

  • To investigate the differentiation potential of human CD14-positive mononuclear cells into endothelial-like cells (ELCs).
  • To characterize the phenotypic and functional properties of these newly formed ELCs.
  • To explore the relationship between monocytic and endothelial cell systems.

Main Methods:

  • Culture of human CD14-positive mononuclear cells with angiogenic growth factors.

Related Experiment Videos

  • Morphological analysis and characterization of cell surface markers using flow cytometry and electron microscopy.
  • Assessment of functional properties, including network formation on fibrin gels and response to vascular endothelial growth factor (VEGF) and tumor necrosis factor-alpha (TNF-alpha).

    • Main Results:

    • CD14 cells transformed into ELCs, exhibiting characteristic endothelial morphology and expressing markers such as von Willebrand factor (vWF), VE-cadherin (CD144), and endoglin (CD105).
    • Electron microscopy revealed structures resembling Weibel-Palade bodies in ELCs, and these cells formed network-like structures in 3D fibrin gels.
    • ELC proliferation and vWF expression were stimulated by VEGF, and adhesion molecules were inducible by TNF-alpha. Dendritic cell markers were not significantly expressed.

    Conclusions:

    • Human CD14-positive mononuclear cells can differentiate into endothelial-like cells under specific culture conditions.
    • These findings suggest a closer relationship between the monocyte/macrophage and endothelial cell systems than previously understood.
    • ELCs derived from monocytes may represent a valuable model for studying endothelial cell biology and vascular development.