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Related Experiment Videos

Evidence for a second function for Saccharomyces cerevisiae Rev1p.

J R Nelson1, P E Gibbs, A M Nowicka

  • 1Department of Biochemistry and Biophysics, School of Medicine and Dentistry, University of Rochester, Rochester, NY 14642, USA.

Molecular Microbiology
|August 10, 2000
PubMed
Summary
This summary is machine-generated.

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The Saccharomyces cerevisiae REV1 gene is crucial for DNA repair. Rev1p has deoxycytidyl transferase activity for abasic site bypass and a second function for UV photoproduct bypass.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The Saccharomyces cerevisiae REV1 gene product, Rev1p, is essential for translesion DNA synthesis and mutagenesis.
  • Rev1p was previously shown to have deoxycytidyl transferase activity, incorporating dCMP opposite abasic sites.

Purpose of the Study:

  • To investigate the dual functions of Rev1p in DNA damage bypass.
  • To characterize the role of Rev1p in bypassing abasic sites and UV photoproducts.

Main Methods:

  • Analysis of yeast mutants (rev1-1) with altered Rev1p activity.
  • Assessing DNA replication and bypass of specific DNA lesions (abasic sites, T-T (6-4) photoproducts).

Main Results:

  • Rev1p is required for abasic site bypass, primarily through dCMP incorporation.

Related Experiment Videos

  • Rev1p also facilitates the bypass of T-T (6-4) UV photoproducts, independent of its deoxycytidyl transferase activity.
  • A rev1-1 mutant retaining deoxycytidyl transferase activity showed impaired bypass of abasic sites, indicating a deficiency in Rev1p's second function.

    • Conclusions:

    • Rev1p possesses at least two distinct functions essential for DNA damage tolerance.
    • One function involves deoxycytidyl transferase activity for abasic site bypass, while a second, distinct function is involved in UV photoproduct bypass.