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Updated: Jul 29, 2026
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
Published on: August 4, 2016
Serial analysis of gene expression: from gene discovery to target identification
1Genzyme Molecular Oncology and Genzyme Corporation, PO Box 9322, Framingham, MA 01701-9322, USA.
Drug Discovery Today
|August 10, 2000
Summary
Serial Analysis of Gene Expression (SAGE) offers comprehensive gene discovery and expression profiling. This open system identifies expressed genes and their levels, unlike closed systems like microarrays.
Area of Science:
- Genomics
- Molecular Biology
- Bioinformatics
Background:
- Traditional gene expression profiling platforms, such as microarrays, operate as 'closed' systems.
- These platforms quantify expression for a limited, predetermined set of genes.
- This limitation restricts comprehensive gene discovery and understanding of complex biological systems.
Purpose of the Study:
- To introduce Serial Analysis of Gene Expression (SAGE) as a powerful genomics tool.
- To highlight SAGE's capabilities in comprehensive gene discovery and quantitative gene expression analysis.
- To differentiate SAGE as an 'open' system compared to 'closed' platforms.
Main Methods:
- Serial Analysis of Gene Expression (SAGE) utilizes a sequence-based approach.
- SAGE analyzes short sequence tags to identify and quantify gene expression.
- This method allows for the discovery of novel genes and transcripts.
Main Results:
- SAGE provides comprehensive gene discovery, identifying a broader range of expressed genes.
- It offers precise quantitative gene expression data.
- SAGE reveals the complete transcriptome, including low-abundance transcripts.
Conclusions:
- SAGE functions as an 'open' system, enabling unbiased gene discovery.
- Its quantitative capabilities facilitate molecular-level characterization of human diseases.
- SAGE serves as a discovery engine for identifying therapeutic targets and diagnostic markers.
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