Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microenvironmental influences on human B-cell development.

F E Bertrand1, C E Eckfeldt, J R Fink

  • 1University of Minnesota Cancer Center, Minneapolis 55455, USA.

Immunological Reviews
|August 10, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<math></math> -event characterization and rejection in point-contact HPGe detectors.

The European physical journal. C, Particles and fields·2022
Same author

First Limit on the Direct Detection of Lightly Ionizing Particles for Electric Charge as Low as e/1000 with the Majorana Demonstrator.

Physical review letters·2018
Same author

Search for Neutrinoless Double-β Decay in ^{76}Ge with the Majorana Demonstrator.

Physical review letters·2018
Same author

The association of abdominal muscle with outcomes after scheduled abdominal aortic aneurysm repair.

Anaesthesia·2017
Same author

Brief Report: Social Support and Coping Strategies of Mothers of Children Suffering from ASD in Kuwait.

Journal of autism and developmental disorders·2017
Same author

Comparison of Gadoterate Meglumine and Gadobutrol in the MRI Diagnosis of Primary Brain Tumors: A Double-Blind Randomized Controlled Intraindividual Crossover Study (the REMIND Study).

AJNR. American journal of neuroradiology·2017
Same journal

Diversity, Equality, and Inclusion in the naïve T Cell Receptor Repertoire.

Immunological reviews·2026
Same journal

Macrophage Plasticity and Immune Remodeling in Ischemic Heart Failure.

Immunological reviews·2026
Same journal

The T Cell Receptor: Molecular Sensor, Therapeutic Mediator and Probabilistic Driver of Adaptive Immunity.

Immunological reviews·2026
Same journal

Tissue-Resident Memory T Cells in the Heart: An Emerging Role in Chronic Inflammation.

Immunological reviews·2026
Same journal

Rethinking Immunity in Tissues: The Biology of Tertiary Lymphoid Structures.

Immunological reviews·2026
Same journal

Inflammation-Driven Lymphoid Structures: Organization, Function, and Clinical Impact Across Autoimmunity, Cancer, and Checkpoint Toxicity.

Immunological reviews·2026
See all related articles

This study shows that human bone marrow stromal cells support B-cell development, guiding cells through key checkpoints and preventing apoptosis. Notch signaling may regulate this crucial B-cell maturation process.

Area of Science:

  • Immunology
  • Developmental Biology
  • Cell Biology

Background:

  • Mammalian B-cell development involves critical checkpoints, including B-cell lineage commitment and immunoglobulin gene rearrangement.
  • The bone marrow (BM) microenvironment significantly influences B-cell maturation.
  • Previous models often rely on transformed cell lines, limiting in vitro study of human B-cell development.

Purpose of the Study:

  • To develop and validate an in vitro model of human B-cell development using non-transformed human BM stromal cells.
  • To investigate the role of the human BM microenvironment in supporting B-cell lineage commitment and progression through developmental checkpoints.
  • To explore the potential involvement of Notch signaling in human B-cell development.

Main Methods:

Related Experiment Videos

  • Utilized freshly isolated, non-transformed human BM stromal cells as an in vitro microenvironment.
  • Co-cultured human CD34+ hematopoietic stem cells with the human BM stromal cell microenvironment.
  • Analyzed B-cell lineage commitment and progression through pre-B-cell receptor (pre-BCR) and B-cell receptor (BCR) checkpoints.
  • Assessed cell survival and expression of Notch receptors and Jagged-1 ligand.

    • Main Results:

    • Human CD34+ cells committed to the B-lineage and successfully navigated pre-BCR and BCR checkpoints within the human BM stromal cell microenvironment.
    • The established microenvironment provided essential survival signals, inhibiting apoptosis in developing B-lineage cells.
    • Differential expression of Notch receptors on B-lineage cells and Jagged-1 on stromal cells was observed, suggesting a role for Notch signaling.

    Conclusions:

    • Freshly isolated human BM stromal cells provide a functional microenvironment for in vitro human B-cell development.
    • This model supports B-cell maturation through key checkpoints and promotes cell survival.
    • Notch signaling is implicated as a potential regulator of human B-cell lineage commitment and checkpoint progression.