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Structural analysis of (methyl-esterified) oligogalacturonides using post-source decay matrix-assisted laser
G J van Alebeek1, O Zabotina, G Beldman
1Wageningen University, Department of Agrotechnology and Food Sciences, Laboratory of Food Chemistry, The Netherlands.
Abstract:
The use of post-source decay matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the structural analysis of ((partly) methyl-esterified) oligogalacturonides (oligoGalA) is described. The fragmentation behavior of purified (un)saturated oligoGalA (degree of polymerization 3-6), methyl-esterified and methyl-glycosydated oligoGalA was studied. General fragmentation patterns are described and used for the elucidation of the positions of methyl esters on partly methyl-esterified oligoGalA. This technique now permits the determination of the position of methyl esters or other substituents on pectic fragments, helping in understanding the mode of action of pectinolytic enzymes.