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Generation of stable recombinant retroviruses containing the beta-globin genes linked to complex regulatory elements
1Department of Biochemistry & Molecular Biology, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.
Molecular Biotechnology
|August 10, 2000
Abstract:
A transient packaging method for a retroviral vector containing beta-globin sequences, mediated by Ca3(PO4)2 precipitation, generated stable recombinant beta-globin retroviruses with intact viral genomes. We suggest the use of this method as an alternative means to overcome the production of beta-globin encoding retroviruses with aberrant viral genomes using the routine packaging procedure.