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T-DNA transfer to maize plants
1IBMP/CNRS, Strasbourg, France. Wen-Hui.Shen@ibmp-ulp.u-strasbg.fr
Molecular Biotechnology
|August 10, 2000
Abstract:
Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on shoots of young maize seedlings after cocultivation with Agrobacterium.