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Micropropagation of Dev-ringal

Bag1, Chandra, Palni

  • 1G.B. Pant Institute of Himalayan Environment and Development, Kosi-Katarmal, 263 643, U.P., Almora, India

Plant Science : an International Journal of Experimental Plant Biology
|August 11, 2000
PubMed
Summary
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A new method efficiently propagates Thamnocalamus spathiflorus bamboo using plant growth regulators. In vitro propagated bamboo plants show comparable physiological functions to seed-raised plants, indicating successful acclimatization.

Area of Science:

  • Plant Biotechnology
  • Plant Physiology
  • Horticulture

Background:

  • Thamnocalamus spathiflorus is an evergreen temperate bamboo species.
  • Efficient in vitro propagation protocols are crucial for mass multiplication and conservation of valuable plant species.
  • Understanding the physiological performance of micropropagated plants post-acclimatization is essential for their successful field establishment.

Purpose of the Study:

  • To develop an efficient in vitro propagation protocol for Thamnocalamus spathiflorus.
  • To evaluate the physiological characteristics, including gas and water vapor exchange rates, of in vitro propagated plants compared to seed-raised plants.

Main Methods:

  • Multiple shoot formation was induced from zygotic embryos and nodal explants using Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA).

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  • Rooting was achieved on a hormone-free medium with a high concentration of IBA.
  • Gas exchange parameters (photosynthesis, stomatal conductance, transpiration, intercellular CO2 concentration) were measured under various light intensities in hardened in vitro propagated plants and seedlings.

    • Main Results:

    • An efficient protocol yielded multiple shoots and 100% rooting.
    • In vitro propagated and hardened plants exhibited light optima for photosynthesis similar to seedlings (approx. 1000 µmol m⁻² s⁻¹).
    • Physiological responses, including water use efficiency, stomatal conductance, and transpiration, were comparable between in vitro propagated and seed-raised plants across different light intensities.

    Conclusions:

    • The developed in vitro propagation protocol is efficient for mass multiplication of Thamnocalamus spathiflorus.
    • In vitro propagated and acclimatized plants are morphologically and functionally comparable to seed-raised plants.
    • This study validates the efficacy of the micropropagation technique for producing genetically similar and physiologically sound bamboo plants.