Urinary excretion of deoxypyridinoline in 24-hour and first-void samples in healthy Turkish children

Area of Science:

• Biochemistry
• Pediatric Endocrinology
• Bone Metabolism

Background:

• Collagen cross-links, like free deoxypyridinoline (f-Dpd), are crucial for bone matrix maturation.
• f-Dpd is a potential biomarker for various bone disorders and can be measured in urine.
• Excretion characteristics of f-Dpd in pediatric populations require further clarification.

Purpose of the Study:

• To investigate urinary f-Dpd excretion in healthy Turkish children using first-void and 24-hour urine samples.
• To evaluate correlations and gender-related differences in f-Dpd excretion between these two sampling methods.
• To determine the feasibility of using first-void urine samples for f-Dpd assessment in pediatric populations.

Main Methods:

• Analyzed urinary f-Dpd in first-void and 24-hour samples from 62 healthy children (31-120 months).
• Utilized the Immulite chemiluminescent technique for sample analysis.
• Assessed correlations and gender differences in f-Dpd excretion relative to creatinine.

Main Results:

• No significant differences in f-Dpd excretion were observed between first-void and 24-hour urine samples.
• A strong positive linear correlation (r=0.77, p<0.05) was found between the two collection methods.
• f-Dpd excretion showed no gender-related differences in either sampling method (p>0.05).

Conclusions:

• First-void urine samples provide a reliable and convenient method for assessing f-Dpd in children.
• This non-invasive approach simplifies bone metabolism monitoring in infants and young children.
• f-Dpd assessment in single voided urine samples is practical for pediatric clinical settings.