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Two pathways for store-mediated calcium entry in human platelets.

S Jenner1, S O Sage

  • 1Department of Physiology, University of Cambridge, UK. sj10014@cus.cam.ac.uk

Platelets
|August 12, 2000
PubMed
Summary
This summary is machine-generated.

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Store-mediated calcium entry in human platelets involves two pathways. An early, tyrosine kinase-independent pathway is followed by a later, tyrosine kinase-dependent pathway, explaining incomplete inhibition by kinase inhibitors.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Physiology

Background:

  • Store-mediated calcium entry is crucial in non-excitable cells like platelets.
  • The precise mechanisms, particularly the role of tyrosine phosphorylation, remain unclear.
  • Previous studies show incomplete inhibition of calcium entry by tyrosine kinase inhibitors.

Purpose of the Study:

  • To investigate the temporal properties of store-mediated calcium entry in human platelets.
  • To elucidate the distinct pathways contributing to calcium influx after store depletion.
  • To determine the specific role of tyrosine kinases in platelet calcium signaling.

Main Methods:

  • Studied store-mediated calcium entry in human platelets over time.
  • Utilized various inhibitors including SKF 96365 and lanthanum (La3+).

Related Experiment Videos

  • Assessed the impact of tyrosine kinase inhibitors on calcium entry dynamics.

    • Main Results:

    • Identified two distinct components of store-mediated calcium entry.
    • An early component is insensitive to SKF 96365, La3+, and tyrosine kinase inhibitors.
    • A later component is inhibited by La3+, SKF 96365, and tyrosine kinase inhibitors.

    Conclusions:

    • Human platelets utilize at least two pathways for store-mediated calcium entry.
    • Tyrosine kinases are involved only in the later stages of this process.
    • This explains the incomplete inhibition observed with tyrosine kinase inhibitors.