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Preliminary study on immunoscintigraphy of a murine lymphoma using technetium-99M labeled chicken antibody
K Subbiah1, U Myleru, M Ananthanarayanan
1Department of Biochemistry, Institute of Bioinorganic and Radiopharmaceutical Chemistry, Forschungszentrum Rossendorf, Dresden, Germany.
Aims And Background:
Radiolabeled antibodies generated against tumor-associated antigens are used for immunoscintigraphy to detect tumors and tumor metastases. Although successful tumor imaging has been achieved using trace-labeled murine monoclonal antibodies, such antibodies often lead to the development of human anti-murine antibodies (HAMA), which limit their subsequent administration for tumor imaging and therapy. It has been reported recently that chicken polyclonal antibodies have high affinity and specificity for the antigen against which they are raised and do not have any immunological cross-reactivity with HAMA.
Methods:
The present study deals with immunoscintigraphy of Dalton's lymphoma, an experimental tumor model using chicken antibodies generated against Dalton's lymphoma-associated antigen (DLAA) and labeled with technetium-99m ((99m)Tc).
Results:
Scintigrams showed specific uptake of the radiolabel resulting in clear tumor images. The radioactivity uptake of the chicken anti-DLAA antibody was about twofold higher than that of the non-specific chicken antibody.
Conclusions:
The results demonstrate the potential of chicken antibody for in vivo radioimmunodetection and localization of tumors.