Structural modifications in the stratum corneum by effect of different solubilizing agents: a study based on...

The action of different solubilizing agents (chloroform-methanol mixtures and the nonionic surfactant octyl glucoside, OG) on the structural organization of the stratum corneum (SC) was investigated by means of the double-layer high-resolution low-temperature scanning electron microscopy technique. Chloroform-methanol mixtures were able to remove mainly the lipid without a significant loss of cohesion in the SC tissue. However, OG treatment caused a partial disaggregation of the corneocytes and their envelopes and, at a macroscopic level, a loss of cohesion in the whole SC tissue. As for lipid domains, after OG treatment the formation of rough structures was detected, probably associated with the disorder in the lipid lamellae. The formation of these new structures could be attributable to the interaction of the lipids with the proteins liberated from the corneocytes. Hence, a direct correlation may be established between the preservation of the structure of the corneocytes and the corneocyte envelope and the cohesion of the whole SC tissue.