Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Phenotypic characterization of CD8(+)NKT cells.

M Emoto1, J Zerrahn, M Miyamoto

  • 1Department of Immunology, Max-Planck-Institute for Infection Biology, Berlin, Germany.

European Journal of Immunology
|August 15, 2000
PubMed
Summary

A novel CD8(+)NKT cell population was identified, primarily in the liver. These cells, distinct from conventional T cells, express unique T cell receptor (TCR) profiles and may play a role in immune regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Receiver circuit improvement of dual frequency-comb ka-band Doppler backscattering system in the large helical device (LHD).

The Review of scientific instruments·2022
Same author

Retroperitoneal fibrosis requiring prompt nephrostomy in a case with immunoglobulin A vasculitis.

Scandinavian journal of rheumatology·2022
Same author

Analysis of the Motional Stark Effect (MSE) diagnostic to measure the rotational transform and current profile in the Large Helical Device.

The Review of scientific instruments·2021
Same author

W-band millimeter-wave back-scattering system for high wavenumber turbulence measurements in LHD.

The Review of scientific instruments·2021
Same author

Retraction: α-GalCer ameliorates listeriosis by accelerating infiltration of Gr-1<sup>+</sup> cells into the liver.

European journal of immunology·2021
Same author

POTENTIAL ADVANTAGE OF REPAGLINIDE MONOTHERAPY IN GLYCEMIC CONTROL IN PATIENTS WITH TYPE 2 DIABETES AND SEVERE RENAL IMPAIRMENT.

Acta endocrinologica (Bucharest, Romania : 2005)·2019

Area of Science:

  • Immunology
  • Cell Biology
  • T cell subsets

Background:

  • Natural Killer T (NKT) cells bridge innate and adaptive immunity.
  • CD8(+) T cells are crucial for cellular immunity.
  • The heterogeneity of NKT cell populations is not fully understood.

Purpose of the Study:

  • To characterize a novel CD8(+)NKT cell population.
  • To investigate the developmental requirements and functional properties of these cells.
  • To determine their relationship to conventional NKT and CD8(+) T cells.

Main Methods:

  • Flow cytometry analysis of T cell receptor (TCR) and activation markers.
  • Assessment of Major Histocompatibility Complex (MHC) class I dependency.
  • Analysis of T cell receptor variable (TCRV) gene usage.

Related Experiment Videos

  • In vitro cytokine production assays (IFN-gamma, IL-4).

    • Main Results:

    • A distinct CD8(+)NKT cell population was found predominantly in the liver, expressing either TCRalpha/beta or TCRgamma/delta.
    • Development of TCRalpha/beta(+) CD8(+)NKT cells depended on MHC class I, unlike TCRgamma/delta(+) counterparts.
    • These cells expressed activation and Ly49 markers, showed specific TCRV gene usage, and produced IFN-gamma upon stimulation.
    • CD8(+)NKT cells were abundant in early life and absent in young athymic mice.

    Conclusions:

    • A unique CD8(+)NKT cell population, distinct from conventional NKT and CD8(+) T cells, has been identified.
    • These cells exhibit developmental plasticity and unique functional characteristics.
    • Their presence, particularly in early life, suggests a potential role in immune regulation.