Partitioning and diffusion of large molecules in fibrous structures
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Updated: Oct 13, 2026

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
1Department of Chemical Engineering, University of Groningen, The Netherlands. j.c.bosma@chem.rug.nl
Abstract:
This paper consists of three parts. In the first part we present diffusion and partition coefficients of proteins in agarose that were measured with gel permeation chromatography. In the second part we present a partition model which includes the effects of solute concentration and of cosolutes. In the third part we modify and extend Ogston's diffusion equation to account for the effects of solute and fiber flexibility, solute concentration and cosolutes. We find good agreement between the proposed models and data from the literature.
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