Redox signaling: globalization of gene expression
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Updated: Oct 13, 2026

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
1Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center, Medical School, 6431 Fannin, Houston, TX 77030, USA.
Abstract:
Here we show that the extent of electron flow through the cbb(3) oxidase of Rhodobacter sphaeroides is inversely related to the expression levels of those photosynthesis genes that are under control of the PrrBA two-component activation system: the greater the electron flow, the stronger the inhibitory signal generated by the cbb(3) oxidase to repress photosynthesis gene expression. Using site-directed mutagenesis, we show that intramolecular electron transfer within the cbb(3) oxidase is involved in signal generation and transduction and this signal does not directly involve the intervention of molecular oxygen. In addition to the cbb(3) oxidase, the redox state of the quinone pool controls the transcription rate of the puc operon via the AppA-PpsR antirepressor-repressor system. Together, these interacting regulatory circuits are depicted in a model that permits us to understand the regulation by oxygen and light of photosynthesis gene expression in R.SPHAEROIDES:
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