[Cyclospora cayetanensis infection. Laboratory diagnosis]
O Vázquez Tsuji1, R Jiménez Domínguez, T Campos Rivera
1Servico de Parasitología y Micología, Universidad Nacional Autónoma de México, D. F.
Revista Latinoamericana De Microbiologia
|August 19, 2000
Summary
Cyclospora cayetanensis causes human gastroenteritis. This review details laboratory diagnostic methods, including direct examination, staining, and microscopy, for accurate identification and differentiation from similar organisms.
Area of Science:
- Medical Parasitology
- Infectious Diseases
- Microbiology
Context:
- Cyclospora cayetanensis, an Apicomplexa protozoan, identified in 1979, causes human gastroenteritis.
- Accurate laboratory diagnosis is crucial for managing Cyclospora infections.
- Distinguishing Cyclospora from similar coccidia and artifacts is a diagnostic challenge.
Purpose:
- To review and emphasize effective laboratory diagnostic methods for Cyclospora cayetanensis.
- To highlight the importance of direct fecal examination with acid-fast stains.
- To discuss morphometric differentiation, sporulation techniques, and microscopic morphology.
Summary:
- Direct examination of fecal matter using acid-fast stains is key for diagnosing Cyclospora cayetanensis.
- Morphometric analysis, sporulation techniques, and histological/electron microscopic examination aid in identification and differentiation.
- The paper details diagnostic experiences and advocates for integrating Cyclospora into diarrheal disease diagnostic protocols.
Impact:
- Enhances the accuracy and efficiency of diagnosing Cyclospora cayetanensis infections.
- Provides a comprehensive guide for clinical laboratories and healthcare institutions.
- Facilitates better patient management and public health strategies for diarrheal diseases.
