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Transfer factors: identification of conserved sequences in transfer factor molecules
1Department of Medicine, University of Colorado Health Sciences Center, Denver, USA.
Molecular Medicine (Cambridge, Mass.)
|August 19, 2000
Summary
Researchers identified a novel peptide sequence (LLYAQDL/VEDN) in transfer factors. While not sufficient for immunity transfer, these peptides block delayed-type hypersensitivity, suggesting a role in target cell binding for transfer factors.
Area of Science:
- Immunology
- Molecular Biology
- Protein Chemistry
Background:
- Transfer factors are small proteins that transfer cell-mediated immunity.
- Purification of individual transfer factors demonstrated their antigen-specificity.
- Transfer factors correct deficient immunity in opportunistic infections and provide prophylaxis.
Purpose of the Study:
- To purify and characterize individual transfer factors.
- To identify specific amino acid sequences within transfer factors.
- To assess the role of identified sequences in transfer factor function.
Main Methods:
- Purification of transfer factors using affinity chromatography and HPLC.
- Sequencing of cyanogen bromide digests.
- Assessment of peptide properties on delayed-type hypersensitivity.
Main Results:
- A novel peptide sequence, LLYAQDL/VEDN, was identified in seven transfer factor preparations.
- These peptides did not transfer delayed-type hypersensitivity.
- Administration of peptides blocked delayed-type hypersensitivity in recipients of native transfer factors without immunosuppression.
Conclusions:
- The identified peptides may represent the target cell-binding portion of transfer factors.
- Further identification of target cells will elucidate transfer factor mechanisms of action.
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