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Relationship between retention, linear velocity and flow for counter-current chromatography
1Brunel Institute for Bioengineering, Brunel University, Uxbridge, Middlesex, UK. ian.sutherland@brunel.ac.uk
Journal of Chromatography. A
|August 19, 2000
Summary
This study links counter-current chromatography retention to mobile phase linear velocity, building on prior work relating retention to flow rate. These findings enable further engineering analysis of fluid dynamics in chromatography.
Area of Science:
- Analytical Chemistry
- Chromatography
- Fluid Dynamics
Background:
- Du et al. demonstrated a correlation between stationary phase retention and the square root of mobile phase flow rate (F(1/2)) in counter-current chromatography.
- This relationship was observed across 12 different stationary phase systems.
Purpose of the Study:
- To establish a connection between the retention observed in Du et al.'s study and the linear velocity of the mobile phase.
- To bridge the gap between chromatographic retention behavior and the kinematics of mobile phase flow.
Main Methods:
- Analysis of existing data from Du et al. [J. Chromatogr. A, 835 (1999) 231].
- Relating chromatographic retention parameters to mobile phase linear velocity.
Main Results:
- A direct relationship was identified between stationary phase retention and the linear velocity of the mobile phase.
- The findings extend the understanding of Du et al.'s results by incorporating fluid dynamics principles.
Conclusions:
- The study successfully links chromatographic retention to mobile phase linear velocity.
- This connection facilitates a deeper engineering analysis of fluid dynamics within chromatographic systems.