[Effect of fat distribution on lipoprotein composition and parameters of lipoperoxidation]

Fat distribution, not overweight and obesity per se, are supposed to be associated with hemodynamic, hemostatic and other metabolic disturbances (insulin resistance, hyperuricemia and dyslipidemia). Moreover, obesity increases the total risk of cardiovascular disease. Oxidative modification of lipoproteins, especially of LDL, is supposed to play a key role in the initiation and progression of atherosclerosis. Therefore we analysed VLDL a LDL composition and Cu(2+)-catalyzed conjugated diene formation in both lipoprotein fractions in patients with intraabdominal fat accumulation and in control group. Patients (33, 12 M/21 F) with intraabdominal fat accumulation (WHCR 1.00 for men, 0.85 for women) revealed, in comparison with control group (72, 47 M/25 F), after adjustment for the same age, increased plasma total cholesterol, apolipoprotein B, non-esterified fatty acid concentrations, and systolic blood pressure as well. In the group of patients increased levels of cholesterol, triglycerides, phospholipids, proteins and apolipoprotein B (only in the fraction of VLDL) were found in the both VLDL and LDL fractions. In this group of patients increased concentrations of conjugated dienes in VLDL and decreased length of lag phase of VLDL were found. Parameters of conjugated diene formation of LDL (basal absorbance, length of lag phase, propagation phase) did not differ significantly from controls. Concomitantly, persons with intraabdominal fat distribution showed decreased titres of antibodies against oxidative modified LDL. The results indicated that the patients with intraabdominal fat accumulation revealed not only adverse composition of VLDL and LDL particles, but also increased VLDL oxidation and oxidability.