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Microbiological methods. Methodology for enteropathogenic Escherichia coli.

I J Mehlman, N T Simon, A C Sanders

    Journal - Association of Official Analytical Chemists
    |March 11, 1975
    PubMed
    Summary
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    Pathogenic Escherichia coli strains show poor growth at temperatures far from host levels. Optimal recovery requires specific media and adjusted temperatures, suggesting modifications to standard analytical methods for improved detection.

    Area of Science:

    • Microbiology
    • Bacteriology

    Background:

    • Pathogenic Escherichia coli biotypes exhibit temperature-dependent growth limitations.
    • Standard microbiological media and incubation temperatures may not be optimal for recovering all pathogenic E. coli strains.

    Purpose of the Study:

    • To evaluate the impact of temperature and different media on the recovery of pathogenic Escherichia coli.
    • To suggest modifications for improved analytical methods for detecting pathogenic E. coli.

    Main Methods:

    • Quantitative recovery of E. coli strains at various temperatures (42.0-45.5°C) in different broths (lauryl tryptose, elevated coliform, MacConkey, enteric enrichment, Gram-negative, nutrient broth, acidified glutamic acid).
    • Assessment of media suitability for small inocula and slow lactose fermenters.
    • Evaluation of motility enhancement in indole-nitrite broth and serological identification methods.

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    Main Results:

    • E. coli recovery decreased significantly at temperatures above 44.0°C, with variations based on the growth medium.
    • Lauryl tryptose and elevated coliform broths supported recovery, while MacConkey and enteric broths were inhibitory.
    • Nutrient broth required carbohydrate for enhanced growth, and standard lactose media failed to recover slow lactose fermenters.

    Conclusions:

    • Standard temperatures and media may require modification for optimal recovery of pathogenic E. coli biotypes.
    • Simplified and supplemented analytical methods, including motility enhancement and specific serological tests, are recommended.
    • Inclusion of Alkalescens-Dispar strains and examination in Shigella sera are necessary for comprehensive identification of dysentery-like biotypes.