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Raman and surface-enhanced Raman scattering spectroscopy of bis-netropsins and their DNA complexes.

M Ermishov1, A Sukhanova, E Kryukov

  • 1Institut Fédératif de Recherche, Biomolécules, UFR de Pharmacie, Université de Reims Champagne-Ardenne, France.

Biopolymers
|August 25, 2000
PubMed
Summary

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Bis-netropsins (bis-Nts) binding to DNA (OLIGs) causes structural changes in both molecules. These DNA structural modifications by bis-Nts may explain their enzyme inhibition activity.

Area of Science:

  • Molecular Biology
  • Biophysics
  • Chemical Biology

Background:

  • Bis-netropsins (bis-Nts) are known inhibitors of DNA-binding enzymes.
  • Understanding the molecular interactions between bis-Nts and DNA is crucial for explaining their inhibitory mechanisms.

Purpose of the Study:

  • To analyze the interactions between bis-netropsins (bis-Nts) and double-stranded oligonucleotides (OLIGs).
  • To investigate the structural changes in bis-Nts and OLIGs upon complexation using spectroscopic methods.
  • To correlate these structural changes with binding affinity and enzyme inhibition.

Main Methods:

  • Raman spectroscopy was employed to monitor structural modifications of bis-Nts and OLIGs.
  • Surface-enhanced Raman scattering (SERS) spectroscopy was used for topological studies of ligand-DNA complexes.

Related Experiment Videos

  • Binding constants were determined to quantify the affinity between bis-Nts and OLIGs.

    • Main Results:

    • Complexation induced conformational changes in both bis-Nts and OLIGs.
    • Structural variations in bis-Nts were dependent on the binding constant, being minimal for specific binding and maximal for nonspecific binding.
    • OLIG conformational changes mirrored the binding constant, indicating bis-Nts perturb DNA structure at binding sites.

    Conclusions:

    • Bis-netropsin binding induces significant structural perturbations in DNA, varying with binding affinity.
    • These observed DNA structural modifications provide a potential explanation for the inhibitory effects of bis-Nts on various DNA-binding enzymes.
    • The study highlights the intricate relationship between ligand structure, DNA conformation, and enzyme inhibition.