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High-throughput screening: new technology for the 21st century
1Molecular Screening Technologies, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA. robert_p_hertzberg@sbphrd.com
Current Opinion in Chemical Biology
|August 26, 2000
Summary
Advancements in high-throughput screening (HTS) technologies are enabling faster, smaller assays. New methods are also improving cell-based screening, addressing downstream bottlenecks.
Area of Science:
- Biotechnology
- Assay Development
- Drug Discovery
Background:
- High-throughput screening (HTS) is crucial for identifying drug candidates.
- Traditional HTS methods face challenges with throughput and assay volume.
- Miniaturization and advanced detection are key areas for improvement.
Purpose of the Study:
- To review recent technological advancements in HTS.
- To highlight innovations in fluorescence, detection, and liquid handling.
- To discuss the impact of new technologies on cell-based assays and downstream processes.
Main Methods:
- Review of recent technological innovations in HTS.
- Analysis of new fluorescence methods and detection platforms.
- Evaluation of advancements in automated liquid handling.
Main Results:
- Significant increases in screening throughput (e.g., 100,000 samples/day).
- Reduction in assay volumes through miniaturization.
- Emerging applications of new technologies in complex cell-based assays.
Conclusions:
- Miniaturized HTS with high throughput is becoming standard.
- New technologies are beginning to alleviate bottlenecks in post-primary screening analysis.
- Continued innovation in HTS is essential for accelerating drug discovery pipelines.