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Laser irradiative tissue probed in situ by collagen 380-nm fluorescence imaging
1Institute for Ultrafast Spectroscopy and Lasers, Departments of Electrical Engineering and Physics, The City College of the City University of New York, New York, NY 10031, USA.
Background And Objective:
There is no ideal method to detect molecular changes in situ of laser-irradiated tissue without removing a section of tissue for histologic examination. A new method is reported to evaluate laser-induced thermal tissue damage in situ by using 380-nm native fluorescence imaging under 340-nm excitation.
Study Design/Materials And Methods:
Native fluorescence imaging was performed on laser irradiated bovine tendon tissue and compared with light illuminated photography and histology with picrosirius red F3BA stain.
Results And Conclusion:
The results indicate that a decrease in collagen fluorescence at 380 nm was observed in laser-induced thermally damaged tissues. The injured region of tissue defined by its fluorescence image coincided with an area defined by photography and histology.