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Capillary electroendoosmotic chromatography of peptides
K Walhagen1, K K Unger, M T Hearn
1Institut fur Anorganische Chemie und Analytische Chemie, Johannes Gutenberg-Universität, Mainz, Germany.
Journal of Chromatography. A
|August 29, 2000
Summary
Capillary electroendoosmotic chromatography (CEC) offers a unique approach for peptide separation, complementing micro-High-Performance Liquid Chromatography (micro-HPLC) and high-performance capillary zone electrophoresis (HPCZE). This review details CEC
Area of Science:
- Analytical Chemistry
- Separation Science
- Biochemistry
Background:
- Peptide separation is crucial for proteomics and biological research.
- Existing techniques like micro-HPLC and HPCZE have limitations.
- Capillary electroendoosmotic chromatography (CEC) offers an orthogonal separation mechanism.
Purpose of the Study:
- To review the current status of peptide separation using CEC.
- To compare CEC with micro-HPLC and HPCZE, highlighting selectivity differences.
- To explore advancements and future potential of CEC in peptide analysis.
Main Methods:
- Review of optimized CEC methods for peptide separation.
- Illustration of peptide separations using n-alkyl bonded silicas and mixed-mode phases.
- Examination of CEC variants, including pressurized CEC (electrically-assisted micro-HPLC).
Main Results:
- CEC provides orthogonal and complementary selectivity to micro-HPLC and HPCZE.
- Current performance limitations of CEC methods are documented.
- Potential for coupling CEC with mass spectrometry for real-time analysis is demonstrated.
Conclusions:
- CEC is a valuable technique for peptide separation, offering unique selectivity.
- Advancements in CEC, including pressurized systems, enhance its applicability.
- Future applications in phenotype/proteomic and zeomic mapping are promising.