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Natural Antioxidant Activity Evaluation Using Electrochemically Generated Superoxide Radical Anion: The SRAGE Assay
Fatah Keffous1, Abdallah Habab2, Mohamed Abbaci3
1Centre de Recherche Scientifique et Technique en Analyses Physico- Chimiques (CRAPC), BP 384, Zone Industrielle, Bou Ismaïl, Tipaza, 42004, RP, Algeria. fkeffous@gmail.com.
A new electrochemical Superoxide Radical Anion Generated Electrochemically (SRAGE) assay offers a biologically relevant method for evaluating antioxidant activity. This novel technique provides accurate antioxidant reducing power classification for natural compounds and complex systems.
Area of Science:
- Electrochemistry
- Biochemistry
- Analytical Chemistry
Background:
- Antioxidant assays rely on parameters like concentration and redox potential for accurate reducing power classification.
- Conventional methods like DPPH and ABTS have limitations in biological relevance compared to superoxide radical anion.
Purpose of the Study:
- Introduce and validate the Superoxide Radical Anion Generated Electrochemically (SRAGE) assay for evaluating antioxidant activity.
- Establish SRAGE as a biologically relevant alternative to existing antioxidant assays.
Main Methods:
- Superoxide radical anion generated electrochemically in situ via controlled reduction.
- Cyclic voltammetry used for controlled superoxide generation and assay validation.
- Scavenging activity of natural antioxidants (rutin, ascorbic acid, quercetin, gallic acid) measured.
Main Results:
- SRAGE assay successfully evaluated antioxidant activity of natural compounds.
- Tested antioxidants showed varying inhibitory activities at 1 mmol L⁻¹.
- Antioxidant reducing power ranking from SRAGE correlated well with DPPH assay results.
Conclusions:
- The SRAGE assay provides a versatile electrochemical platform for antioxidant activity evaluation.
- SRAGE is suitable for assessing pure compounds, biological extracts, and various formulations.
- This method offers a distinct advantage over conventional discoloration assays due to biological relevance.
