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Published on: May 16, 2014
Liquid Chromatographic Enantioseparation of Some Fluoroquinoline Drugs Using Several Polysaccharide-Based Chiral
Mohamed Nadjib Rebizi1, Khaled Sekkoum1, Nasser Belboukhari1
1Bioactive Molecules & Chiral separation Laboratory, University of Bechar, Bechar, Algeria.
Abstract:
The enantioseparation of three fluoroquinoline antibacterial drugs, namely, flumequine, ofloxacin and lomefloxacin using high-performance liquid chromatography was optimized on seven polysaccharide-derived chiral stationary phases, namely, Chiralpak® IB, chiralpak® IA, Chiralpak® AD, Chiralcel® OJ, Chiralcel® OD, Chiralcel® OD-H and Chiralcel® OZ-3 and applying different mobile phases in isocratic mode is described. The role of addition of organic additives was also investigated. A baseline separation of flumequine, ofloxacin and lomefloxacin enantiomers was achieved. Parameters influencing enantioseparation including mobile phase, organic additive and chemical nature of the chiral selector found to be highly influencing on the enantiomeric separation were investigated. Chiral recognition mechanism(s) are also presented.
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