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11:20
Fabrication and Visualization of Capillary Bridges in Slit Pore Geometry
Published on: January 9, 2014
Modelling of the pore flow in capillary electrochromatography
1Polymer Analysis Group, Department of Chemical Engineering, University of Amsterdam, The Netherlands.
Journal of Chromatography. A
|August 29, 2000
Summary
Pore flow in capillary electrochromatography (CEC) significantly impacts separation efficiency. This study shows pore flow in silica particles can be reliably predicted using the Rice and Whitehead expression, improving CEC performance.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrochromatography (CEC) relies on fluid flow through porous particles.
- Understanding pore flow is crucial for optimizing CEC efficiency in both interactive and exclusion modes.
Purpose of the Study:
- To investigate pore flow in porous silica particles used in CEC.
- To compare experimental migration data with theoretical predictions of pore flow.
Main Methods:
- Measured migration behavior of polystyrene standards in di-methylformamide (DMF) with varying lithium chloride concentrations.
- Employed a theoretical model based on cylindrical pores and the Rice and Whitehead expression for flow calculation.
- Utilized porosimetry data to characterize pore diameters.
Main Results:
- Observed reasonable to good agreement between experimental and predicted data when assuming series pore arrangements.
- Found that flow in 100 Å pores can be substantial compared to interstitial flow, particularly at 10 mmol/l ionic strength.
Conclusions:
- Pore flow within porous particles in CEC is a critical factor for separation efficiency.
- The Rice and Whitehead expression provides a reliable method for predicting pore flow in CEC systems.
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