Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modelling of the pore flow in capillary electrochromatography.

R Stol1, H Poppe, W T Kok

  • 1Polymer Analysis Group, Department of Chemical Engineering, University of Amsterdam, The Netherlands.

Journal of Chromatography. A
|August 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Keratinocytes and neutrophils are important sources of proinflammatory molecules in hidradenitis suppurativa.

The British journal of dermatology·2015
Same author

Influence of temperature on vibrational spectra and consequences for the predictive ability of multivariate models.

Analytical chemistry·2011
Same author

Theoretical description of the influence of external radial fields on the electroosmotic flow in capillary electrophoresis.

Analytical chemistry·2011
Same author

Loteprednol etabonate: a soft steroid for the treatment of allergic diseases of the airways.

Drugs of today (Barcelona, Spain : 1998)·2003
Same author

Mass transfer in rectangular chromatographic channels.

Journal of chromatography. A·2003
Same author

Position and intensity of system (eigen) peaks in capillary zone electrophoresis.

Journal of chromatography. A·2002
Same journal

Portable cold-assisted head-space solid-phase microextraction coupled with GC-MS/MS for sensitive determination of trace polychlorinated naphthalenes in water.

Journal of chromatography. A·2026
Same journal

Characterization of phosphorous impurities originating from the synthesis of Sarin.

Journal of chromatography. A·2026
Same journal

Extraction and chromatographic purification of purpurin: A scalable approach using modified dry column vacuum chromatography.

Journal of chromatography. A·2026
Same journal

Development and validation of a modified QuEChERS-LC-MS/MS method for multi-class pesticide residue analysis in soils from pesticide production sites.

Journal of chromatography. A·2026
Same journal

Acid-treated ZIF-67-derived porous carbon based QuEChERS coupled with LC-MS/MS for sensitive determination of multi-pesticide residues.

Journal of chromatography. A·2026
Same journal

Polybenzimidazole-based magnetic solid-phase extraction coupled with gas chromatography-mass spectrometry for the determination of triazine herbicides in environmental waters.

Journal of chromatography. A·2026
See all related articles

Pore flow in capillary electrochromatography (CEC) significantly impacts separation efficiency. This study shows pore flow in silica particles can be reliably predicted using the Rice and Whitehead expression, improving CEC performance.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) relies on fluid flow through porous particles.
  • Understanding pore flow is crucial for optimizing CEC efficiency in both interactive and exclusion modes.

Purpose of the Study:

  • To investigate pore flow in porous silica particles used in CEC.
  • To compare experimental migration data with theoretical predictions of pore flow.

Main Methods:

  • Measured migration behavior of polystyrene standards in di-methylformamide (DMF) with varying lithium chloride concentrations.
  • Employed a theoretical model based on cylindrical pores and the Rice and Whitehead expression for flow calculation.
  • Utilized porosimetry data to characterize pore diameters.

Related Experiment Videos

Main Results:

  • Observed reasonable to good agreement between experimental and predicted data when assuming series pore arrangements.
  • Found that flow in 100 Å pores can be substantial compared to interstitial flow, particularly at 10 mmol/l ionic strength.

Conclusions:

  • Pore flow within porous particles in CEC is a critical factor for separation efficiency.
  • The Rice and Whitehead expression provides a reliable method for predicting pore flow in CEC systems.