Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved column preparation and performance in capillary electrochromatography.

E Rapp1, E Bayer

  • 1Research Center of Nucleic Acid and Peptide Chemistry, Institut für Organische Chemie, Universität Tübingen, Germany.

Journal of Chromatography. A
|August 29, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surgical denervation of the thumb CMC joint: anatomy, technique.

Hand surgery & rehabilitation·2021
Same author

Proteotyping of laboratory-scale biogas plants reveals multiple steady-states in community composition.

Anaerobe·2017
Same author

Bilateral carpal tunnel surgery in one operation: Retrospective study.

Hand surgery & rehabilitation·2016
Same author

Proteotyping of biogas plant microbiomes separates biogas plants according to process temperature and reactor type.

Biotechnology for biofuels·2016
Same author

Sialic acid-specific affinity chromatography for the separation of erythropoietin glycoforms using serotonin as a ligand.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences·2016
Same author

Sample prefractionation with liquid isoelectric focusing enables in depth microbial metaproteome analysis of mesophilic and thermophilic biogas plants.

Anaerobe·2013
Same journal

Retention prediction in reversed-phase liquid chromatography using XGBoost-based quantitative structure-retention relationships models.

Journal of chromatography. A·2026
Same journal

Impurity profiling of lipid-conjugated oligonucleotides using reversed-phase with and without ion-pair reagents and hydrophilic interaction liquid chromatography.

Journal of chromatography. A·2026
Same journal

Preparation of magnetic zwitterionic covalent organic frameworks for rapid simultaneous extraction of hydrophilic and hydrophobic organophosphates from environmental waters coupled with UHPLC-MS/MS determination.

Journal of chromatography. A·2026
Same journal

Analysis of organic and inorganic acids in biomass pyrolysis process samples by ion chromatography-mass spectrometry.

Journal of chromatography. A·2026
Same journal

Separation and enrichment of phages at the interface between two phases in a green solvent-based sugaring-out extraction system.

Journal of chromatography. A·2026
Same journal

Advances and perspectives in Oligo(dT) Affinity chromatography for mRNA capture: Resins, ligands and process intensification.

Journal of chromatography. A·2026
See all related articles

New capillary columns overcome common issues in capillary electrochromatography (CEC) and capillary high-performance liquid chromatography (HPLC). These improved columns offer enhanced reproducibility and longevity for analytical applications.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) faces challenges including column manufacturing variability, bubble formation, and limited column lifetimes.
  • A restricted choice of packing particles further hinders the widespread adoption and reproducibility of CEC methods.

Purpose of the Study:

  • To develop novel fritless or single-frit, internally tapered, segmented, and dead-volume free coupled capillary columns.
  • To address the limitations of conventional capillary columns used in CEC and capillary HPLC.

Main Methods:

  • Fabrication of internally tapered, segmented capillary columns with fritless or single-frit designs.
  • Performance evaluation using Van Deemter plots to assess chromatographic efficiency and separation quality.
  • Application testing in both capillary electrochromatography and capillary high-performance liquid chromatography.

Related Experiment Videos

Main Results:

  • The developed columns demonstrate high quality and overcome common CEC problems like non-reproducible manufacture and bubble formation.
  • The fritless/single-frit and dead-volume free coupled designs contribute to improved column performance and extended lifetimes.
  • Van Deemter analysis confirmed the efficiency of these novel capillary columns.

Conclusions:

  • The new column designs offer a viable solution to persistent problems in capillary chromatography.
  • These high-quality capillaries are suitable for both CEC and capillary HPLC, enhancing their practical utility.
  • The advancements promote greater reproducibility and reliability in chromatographic separations.