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Decrease of neuronal nitric oxide synthase in the cerebellum of aged rats.

W Yu1, S Juang, J Lee

  • 1Department of Pharmacology, College of Medicine, National Cheng Kung University, Taiwan 70101, Republic of, Tainan City, China.

Neuroscience Letters
|August 30, 2000
PubMed
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Neuronal nitric oxide synthase (nNOS) expression and activity decrease in the aging rat cerebellum. This age-related decline in nNOS may contribute to central nervous system (CNS) dysfunction in older mammals.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Aging Research

Background:

  • Nitric oxide (NO) acts as a crucial neurotransmitter in the central nervous system (CNS).
  • Dysregulation of NO signaling is implicated in various pathophysiological processes.
  • Understanding age-related changes in NO production is vital for comprehending CNS aging.

Purpose of the Study:

  • To investigate age-dependent alterations in neuronal nitric oxide synthase (nNOS) in the rat cerebellum.
  • To correlate changes in nNOS expression and activity with aging.

Main Methods:

  • Utilized Northern blot to assess nNOS mRNA levels in Wistar rats across different age groups (2-24 months).
  • Employed Western blot analysis to quantify nNOS protein expression.
  • Measured nNOS activity by determining the conversion rate of L-arginine to L-citrulline.

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Main Results:

  • A significant reduction in nNOS mRNA and protein levels was observed in 6, 12, and 24-month-old rats compared to 2-month-old rats.
  • nNOS activity showed a significant decrease only in the oldest group (24 months).

Conclusions:

  • Aging in rats is associated with decreased expression of nNOS in the cerebellum.
  • The observed decline in nNOS may underlie age-related neurological dysfunction in mammals.