Related Experiment Video
Updated: Aug 1, 2026

In vivo Calcium Imaging of Mouse Geniculate Ganglion Neuron Responses to Taste Stimuli
Published on: February 11, 2021
Adenosine triphosphate mobilizes cytosolic calcium and modulates ionic currents in mouse taste receptor cells
Y V Kim1, Y V Bobkov, S S Kolesnikov
1Institute of Cell Biophysics, Russian Academy of Sciences, Moscow Region, 142290, Pushchino, Russia.
Abstract:
By using photometry and the patch clamp technique, we identified P(2Y)-like receptors in mouse taste receptor cells (TRCs) and found them to be coupled to Ca(2+) mobilization and ionic current modulation. Particularly, adenosine triphosphate (ATP) and the P(2Y) agonist 2-methylthio-ATP increased intracellular Ca(2+) by stimulating the phosphoinositide pathway, whereas beta, gamma-methylene-D-ATP, a P(2X) agonist, was ineffective. In a distinctive TRC subpopulation, ATP closed Ca(2+) channels. This regulation may underlie the negative feedback tuning neurotransmitter release. By mobilizing intracellular Ca(2+), ATP activated Ca(2+)-dependent Cl(-) channels, the intracellular event that may universally occur upon taste stimulation triggering IP(3) formation and Ca(2+) release in the TRC cytoplasm.
Related Concept Videos
G-Protein Gated Ion Channels
Sensory organs,...
IP3/DAG Signaling Pathway
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Ligand-Gated Ion Channel Receptor: Gating Mechanism
The Physiology of Taste

