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Related Experiment Videos

Pulmonary microvascular changes during sepsis: evaluation using intravital videomicroscopy.

D G McCormack1, S Mehta, K Tyml

  • 1The A.C. Burton Vascular Biology Laboratory, London Health Sciences Centre-Victoria Campus, London, Ontario, Canada.

Microvascular Research
|August 31, 2000
PubMed
Summary

Sepsis impairs pulmonary arteriole function and increases leukocyte adhesion and protein leakage in rat lungs. This study visualizes these microvascular changes in vivo, revealing reduced contractility to phenylephrine and hypoxia.

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Area of Science:

  • Pulmonary circulation research
  • Sepsis pathophysiology
  • Microvascular dynamics

Background:

  • Sepsis induces pulmonary microvascular alterations, including abnormal reactivity, leukocyte sequestration, and alveolar protein leakage.
  • Previous studies suggest these changes but lack direct in vivo visualization of pulmonary arterioles.

Purpose of the Study:

  • To develop and utilize a method for direct in vivo assessment of pulmonary microcirculation changes in a rat model of sepsis.
  • To investigate alterations in vascular reactivity, leukocyte traffic, and alveolar capillary permeability during sepsis.

Main Methods:

  • Developed an intravital videomicroscopy technique in rats with a transparent thoracic window for lung visualization.
  • Induced sepsis via cecal ligation and perforation, then assessed arteriolar contractility to phenylephrine (PE) and hypoxia.

Related Experiment Videos

  • Quantified leukocyte traffic using Rhodamine labeling and albumin leak using FITC-labeled albumin.

    • Main Results:

    • Septic rats showed attenuated vascular contractility in both small and medium pulmonary arterioles in response to PE.
    • Medium-sized arterioles in septic rats exhibited hypocontractility to hypoxia.
    • Increased numbers of stationary leukocytes and enhanced alveolar capillary protein leak were observed in septic lungs.

    Conclusions:

    • Direct in vivo visualization of pulmonary microvascular responses to hypoxia and PE is feasible.
    • Pulmonary arterioles display attenuated contractile responses to PE and hypoxia in sepsis, consistent with in vitro findings.
    • Sepsis leads to increased leukocyte sequestration and protein leakage in the pulmonary microcirculation.