Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thin sections for hard tissue histology: a new procedure.

S Caropreso1, L Bondioli, D Capannolo

  • 1School of Dentistry, Department of Experimental Medicine and Biological Science, University of Rome 'Tor Vergata', via di Tor Vergata 135, 00133 Rome, Italy. Anthropology, P.

Journal of Microscopy
|September 6, 2000
PubMed
Summary

This study presents a simplified histological preparation method for teeth and bones using dental materials. This technique improves section quality and significantly reduces processing time for histological analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Different Mutational Profiles of Subcutaneous Panniculitis-like T-cell Lymphoma and Lupus Panniculitis: An Additional Case Series.

Actas dermo-sifiliograficas·2024
Same author

Different Mutational Profiles of Subcutaneous Panniculitis-like T-cell Lymphoma and Lupus Panniculitis: An Additional Case Series.

Actas dermo-sifiliograficas·2024
Same author

Classifications of cutaneous lymphomas and lymphoproliferative disorders: An update from the EORTC cutaneous lymphoma histopathology group.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Correction: 'Dental cementum virtual histology of Neanderthal teeth from Krapina (Croatia, 130-120 kyr): an informed estimate of age, sex and adult stressors' (2022), by Cerrito <i>et al.</i>

Journal of the Royal Society, Interface·2024
Same author

Recent advances in cutaneous lymphoma-implications for current and future classifications.

Virchows Archiv : an international journal of pathology·2022
Same author

[Orchiectomy due to early syphilis].

Urologie (Heidelberg, Germany)·2022

Area of Science:

  • Paleontology
  • Materials Science
  • Histology

Background:

  • Histological analysis of teeth and bones requires precise sectioning.
  • Conventional methods for preparing thin sections can be time-consuming and complex.
  • Improving section quality and reducing preparation time are crucial for scientific research.

Purpose of the Study:

  • To introduce a simplified embedding-cutting-mounting procedure for thin sections of teeth and bones.
  • To enhance the quality of histological sections.
  • To reduce the time and steps required for specimen preparation.

Main Methods:

  • Utilized a dental adhesive system, composite resins, and conventional embedding resins.
  • Developed a detailed embedding-cutting-mounting protocol.

Related Experiment Videos

  • Focused on accurate sample positioning within the resin to prevent section damage.

    • Main Results:

    • Achieved thin sections of approximately 100 micrometers.
    • Prevented cracks and distortions during the cutting phase.
    • Improved the overall quality of mounting sections on slides.
    • Substantially reduced the steps and time needed for histological analysis.

    Conclusions:

    • The proposed dental materials-based system offers a simple and effective method for preparing thin sections of teeth and bones.
    • This technique enhances section quality and streamlines the histological analysis process.
    • The method is applicable to both modern and archaeological specimens.