Microsatellite DNA variants between the inbred SENCAR mouse strains

Area of Science:

• Carcinogenesis and Tumor Biology
• Genetics and Genomics
• Animal Models in Research

Background:

• The two-stage mouse skin carcinogenesis model using 7,12-dimethylbenz[a]anthracene and 12-O-tetradecanoylphorbol-13-acetate is crucial for studying genetic susceptibility to carcinogens.
• Outbred SENCAR mice are widely used for this model, developing squamous cell carcinomas (SCCs) from papillomas, serving as a model for epithelial tumorigenesis and malignant progression.
• Nine recently developed inbred SENCAR strains exhibit varying sensitivities to carcinogenesis, including dissociation between papilloma development and SCC conversion.

Purpose of the Study:

• To address the limited genetic mapping utility of new SENCAR-derived inbred strains due to a lack of DNA variant information.
• To analyze DNA variants within nine inbred SENCAR strains using microsatellite markers.
• To provide essential data for future genetic analyses and the development of congenic strains.

Main Methods:

• Analysis of nine inbred SENCAR mouse strains.
• Utilized microsatellite markers distributed across all 20 mouse chromosomes.
• Genotyping to identify variant alleles among the strains.

Main Results:

• Identified and reported variant alleles across the 20 chromosomes for the nine inbred SENCAR strains.
• Established a molecular basis for differentiating these closely related strains.
• Provided a comprehensive catalog of genetic variations within the SENCAR-derived inbred lines.

Conclusions:

• The identified DNA variant alleles are crucial for distinguishing between the nine inbred SENCAR strains.
• This genetic information will facilitate linkage analysis and the development of marker-assisted congenic strains.
• The findings enhance the utility of these SENCAR strains for genetic studies of carcinogenesis and tumor progression.