Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Docking domains and substrate-specificity determination for MAP kinases.

A D Sharrocks1, S H Yang, A Galanis

  • 1School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester, UK M13 9PT. a.d.sharrocks@man.ac.uk

Trends in Biochemical Sciences
|September 6, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Association between residential greenness exposure and obesity risk based on remote sensing].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2026
Same author

Application of artificial intelligence on magnetocardiology.

Clinical hemorheology and microcirculation·2026
Same author

Effects of partial replacement of soybean meal by flaxseed cake on growth performance, rumen function and meat quality of Simmental cows.

Animal : an international journal of animal bioscience·2026
Same author

Multifunctional Primer for Dentin Bonding via Biomimetic Mineralization.

Journal of dental research·2025
Same author

[Clinical analysis of 8 cases of laparoscopic combined with colonoscopic transanal total mesorectal resection].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2025
Same author

[Efficacy of Naldemedine in opioid-induced constipation in cancer patients].

Zhonghua yi xue za zhi·2025
Same journal

Peptideins: Navigating the gray zone of the proteome.

Trends in biochemical sciences·2026
Same journal

A metabolon channels nicotine biosynthesis.

Trends in biochemical sciences·2026
Same journal

Better call chaperone.

Trends in biochemical sciences·2026
Same journal

Biochemistry at scale: Seeing both the forest and the trees.

Trends in biochemical sciences·2026
Same journal

Voices across Asia and Oceania: Biochemistry across borders.

Trends in biochemical sciences·2026
Same journal

Metabolic control of RNA splicing by polyamines.

Trends in biochemical sciences·2026
See all related articles

Mitogen-activated protein (MAP) kinases use docking domains to bind substrates, ensuring specific cell signalling. Additional substrate features further enhance the accuracy of MAP kinase phosphorylation events.

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Eukaryotic cells rely on precise signalling pathways for proper function.
  • Mitogen-activated protein (MAP) kinases are crucial regulators of cellular processes.
  • Ensuring specificity in kinase-substrate interactions is vital for signal transduction fidelity.

Purpose of the Study:

  • To elucidate the role of docking domains in MAP kinase specificity.
  • To investigate how docking interactions enhance substrate phosphorylation.
  • To identify additional substrate-specific determinants in MAP kinase signalling.

Main Methods:

  • Utilizing biochemical assays to study protein-protein interactions.
  • Employing molecular biology techniques to analyze kinase-substrate binding.

Related Experiment Videos

  • Investigating substrate phosphorylation kinetics in vitro.

    • Main Results:

    • Docking domains were confirmed as key mediators of MAP kinase substrate recognition.
    • These domains significantly increase the fidelity and efficiency of kinase action.
    • Further substrate-specific elements were identified that refine phosphorylation events.

    Conclusions:

    • Docking domains are essential for ensuring specific MAP kinase signalling.
    • Substrate-based determinants provide an additional layer of regulation for kinase activity.
    • These mechanisms collectively maintain the integrity of eukaryotic cell signalling pathways.