Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The presence of multiple rat DSP-PP transcripts.

H H Ritchie1, L Wang

  • 1Department of Cardiology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109-1078, USA. helenar@umich.edu

Biochimica Et Biophysica Acta
|September 9, 2000
PubMed
Summary

Researchers discovered a new variant of phosphophoryn (PP) in rat dentin, which is a smaller protein with reduced negative charge. This finding suggests multiple PP variants may influence dentin mineralization processes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of EDTA on the hydration mechanism of mineral trioxide aggregate.

Journal of dental research·2007
Same author

Dentin-pulp complex responses to carious lesions.

Caries research·2006
Same author

Development of an odontoblast in vitro model to study dentin mineralization.

Connective tissue research·2005
Same author

Immunohistochemical characterization of rapid dentin formation induced by enamel matrix derivative.

Calcified tissue international·2004
Same author

Effects of dexamethasone, vitamin A and vitamin D3 on DSP-PP mRNA expression in rat tooth organ culture.

Biochimica et biophysica acta·2004
Same author

The conservation and regulation of rat DSP-PP gene.

Connective tissue research·2002

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biomineralization

Background:

  • Phosphoproteins (PPs) are abundant non-collagenous proteins (NCPs) in dentin, crucial for mineralization.
  • Dentin sialoprotein (DSP) and PP genes are located together (DSP-PP) in mammals.
  • Previous studies indicated multiple DSP-PP transcripts in rat incisors.

Purpose of the Study:

  • To investigate the nature of multiple DSP-PP transcripts in rat dentin.
  • To characterize a novel PP cDNA variant.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze RNA.
  • cDNA sequencing was performed to identify PP variants.

Main Results:

  • A PP cDNA variant encoding a 171-amino acid peptide (PP(171)) was identified.

Related Experiment Videos

  • PP(171) shares characteristics with the known PP(240) but has a reduced size and negative charge.
  • Evidence supports the existence of multiple DSP-PP transcripts with varying PP sequences in rats.

    • Conclusions:

    • Rat dentin contains multiple DSP-PP transcripts encoding PP variants of different sizes.
    • The identified PP(171) variant may differentially impact dentin mineralization due to its altered properties.