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Aggregation studies of humic acid using multiangle laser light scattering.

T J Manning¹, T Bennett, D Milton

¹Department of Chemistry, Valdosta State University, GA 31698, USA. tmanning@valdosta.edu

The Science of the Total Environment

|September 16, 2000

Summary

This study measured the size and molecular weight of humic acid (HA), a component of dissolved organic carbon. Results show HA has a large average molecular mass and RMS radius in aqueous solutions.

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Area of Science:

Environmental Chemistry
Polymer Science

Background:

Naturally occurring humic acid (HA) is a significant component of dissolved organic carbon (DOC) in aquatic environments.
Understanding the physicochemical properties of HA is crucial for environmental processes and water quality assessment.

Purpose of the Study:

To determine the average molecular weight and root-mean-square (RMS) radius of humic acid in an aqueous phase.
To characterize the size and mass distribution of naturally occurring humic acid.

Main Methods:

Utilized multiangle laser light scattering (MALLS) for precise measurements.
Employed Zimm plot analysis with Berry Formulism and a second-order fit for data interpretation.

Main Results:

Determined an average molar mass of 1.164 x 10(8) g/mol for humic acid.

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Measured an average RMS radius (Rz) of 436.0 +/- 36 nm at 25 degrees C.

Conclusions:

Humic acid in aqueous solution exhibits a substantial molecular size and mass.
MALLS provides a robust method for characterizing complex natural organic matter like humic acid.