Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sorbic acid resistance: the inoculum effect.

H Steels1, S A James, I N Roberts

  • 1Microbiology Section, Unilever Research, Colworth House, Sharnbrook, Bedford MK44 1LQ, UK.

Yeast (Chichester, England)
|September 19, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intestinal microbiota profiles of captive-bred cynomolgus macaques reveal influence of biogeography and age.

Animal microbiome·2025
Same author

Visualising coordination chemistry: fluorescence X-ray absorption near edge structure tomography.

Chemical communications (Cambridge, England)·2016
Same author

Rapid identification of the genus Dekkera/Brettanomyces, the Dekkera subgroup and all individual species.

International journal of food microbiology·2014
Same author

X-ray fluorescence imaging reveals subcellular biometal disturbances in a childhood neurodegenerative disorder.

Chemical science·2014
Same author

A glycoprotein associated with the acquisition of the self-incompatibility system by maturing stigmas of Brassica oleracea.

Planta·2013
Same author

Pollen-pistil interaction in Brassica oleracea : Events prior to pollen germination.

Planta·2013
Same journal

Comparative Analysis of Stress Adaptation in the Yeast Microbiome of Cactus.

Yeast (Chichester, England)·2026
Same journal

Enhanced Production of Recombinant Thermophilic Xylanase X11P in Ogataea polymorpha via In-Silico Signal Peptide Discovery and Fed-Batch Fermentation.

Yeast (Chichester, England)·2026
Same journal

Sugar Metabolisms Altered By Undissociated Forms of Organic Acids Based on the Emergence of [GAR<sup>+</sup>] Cells in Saccharomyces cerevisiae.

Yeast (Chichester, England)·2026
Same journal

Methods to Study Mitochondrial Metabolism and Homeostasis in Fission Yeast.

Yeast (Chichester, England)·2026
Same journal

Genetic Tools in the Nakaseomyces clade for Evolutionary Comparisons of Signal Transduction Pathways.

Yeast (Chichester, England)·2026
Same journal

rDNAmine: A New Tool for the Analysis of Long Repetitive Sequences.

Yeast (Chichester, England)·2026
See all related articles

Spoilage yeast Zygosaccharomyces bailii exhibits significant resistance to sorbic acid, a common food preservative. This resistance is not heritable but due to a small number of phenotypically resistant cells within the population.

Area of Science:

  • Food microbiology
  • Yeast genetics
  • Antimicrobial resistance

Background:

  • Zygosaccharomyces bailii is a spoilage yeast known for its osmotolerance, fermentative ability, and resistance to preservatives.
  • High concentrations of common food preservatives like sorbic acid are often required to inhibit Zygosaccharomyces bailii growth.
  • An inoculum effect, where higher cell densities require greater antimicrobial concentrations, has been observed with Zygosaccharomyces bailii and sorbic acid.

Purpose of the Study:

  • To investigate the cause of the inoculum effect observed with sorbic acid and Zygosaccharomyces bailii.
  • To determine if the resistance to sorbic acid is heritable or due to phenotypic variation within the yeast population.

Main Methods:

  • Testing the inoculum effect of sorbic acid against Zygosaccharomyces bailii NCYC 1766.

Related Experiment Videos

  • Investigating potential causes for the inoculum effect, including metabolic degradation, adsorption, cell-cell signaling, and growth time.
  • Analyzing the diversity of sorbic acid resistance in individual Zygosaccharomyces bailii cells using microtitre plates.
  • Employing 26S ribosomal DNA sequencing to identify genetic differences between resistant and susceptible cells.
  • Assessing the heritability of sorbic acid resistance by re-inoculating 'super cells'.

    • Main Results:

    • A substantial inoculum effect was confirmed for sorbic acid against Zygosaccharomyces bailii.
    • The inoculum effect was not attributed to sorbic acid metabolism, adsorption, cell-cell signaling, or insufficient growth time.
    • Individual Zygosaccharomyces bailii cells displayed significant variation in sorbic acid resistance.
    • 26S ribosomal DNA sequencing revealed no genetic distinctions between highly resistant ('super') cells and the general population.
    • The resistance phenotype was not heritable, and 'super cells' did not represent a genetically distinct subpopulation.

    Conclusions:

    • The inoculum effect observed with sorbic acid and Zygosaccharomyces bailii is attributed to the presence of a small number of phenotypically resistant cells.
    • Sorbic acid resistance in Zygosaccharomyces bailii is a non-heritable trait, arising from phenotypic diversity within the population.