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RNAi Trigger Delivery into Anopheles gambiae Pupae

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Yarrowia lipolytica SRP receptor alpha-subunit.

K Kim¹, D M Ogrydziak

¹Institute of Marine Resources, University of California, One Shields Avenue, Davis, CA 95616, USA.

Yeast (Chichester, England)

|September 19, 2000

Summary

Researchers identified the Yarrowia lipolytica SRP101 gene, crucial for the signal recognition particle (SRP) receptor alpha-subunit. This gene is vital for cell growth and protein translocation, though not essential.

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Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

The signal recognition particle (SRP) pathway is essential for targeting proteins to membranes.
Understanding the components of this pathway in diverse organisms like Yarrowia lipolytica is key to elucidating protein translocation mechanisms.

Purpose of the Study:

To clone and characterize the Yarrowia lipolytica homologue of the SRP receptor alpha-subunit (SRP101).
To investigate the functional role of Y. lipolytica SRP101 in protein synthesis and translocation.

Main Methods:

Degenerate primers targeting conserved GTP-binding domains were used for cloning.
DNA sequencing was performed to determine the gene's open reading frame and protein characteristics.
Conditional mutations were created to assess the gene's essentiality and functional impact.

Main Results:

The Y. lipolytica SRP101 gene was cloned, encoding a 557-amino acid protein with a conserved GTP-binding site.
Sequence identity was found with homologous proteins from S. cerevisiae, mammals, and E. coli.
SRP101 is important but not essential for cell growth; mutations affected alkaline extracellular protease and Kar2p synthesis/translocation.

Conclusions:

Y. lipolytica SRP101 functions as an SRP receptor subunit, playing a role in protein targeting.
The findings contribute to understanding the conserved nature and functional importance of the SRP pathway across different species.