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Yarrowia lipolytica SRP receptor alpha-subunit.
1Institute of Marine Resources, University of California, One Shields Avenue, Davis, CA 95616, USA.
Yeast (Chichester, England)
|September 19, 2000
Summary
Researchers identified the Yarrowia lipolytica SRP101 gene, crucial for the signal recognition particle (SRP) receptor alpha-subunit. This gene is vital for cell growth and protein translocation, though not essential.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- The signal recognition particle (SRP) pathway is essential for targeting proteins to membranes.
- Understanding the components of this pathway in diverse organisms like Yarrowia lipolytica is key to elucidating protein translocation mechanisms.
Purpose of the Study:
- To clone and characterize the Yarrowia lipolytica homologue of the SRP receptor alpha-subunit (SRP101).
- To investigate the functional role of Y. lipolytica SRP101 in protein synthesis and translocation.
Main Methods:
- Degenerate primers targeting conserved GTP-binding domains were used for cloning.
- DNA sequencing was performed to determine the gene's open reading frame and protein characteristics.
- Conditional mutations were created to assess the gene's essentiality and functional impact.
Main Results:
- The Y. lipolytica SRP101 gene was cloned, encoding a 557-amino acid protein with a conserved GTP-binding site.
- Sequence identity was found with homologous proteins from S. cerevisiae, mammals, and E. coli.
- SRP101 is important but not essential for cell growth; mutations affected alkaline extracellular protease and Kar2p synthesis/translocation.
Conclusions:
- Y. lipolytica SRP101 functions as an SRP receptor subunit, playing a role in protein targeting.
- The findings contribute to understanding the conserved nature and functional importance of the SRP pathway across different species.
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